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Set de Primers e Sonda VIC/HEX
- Vendedor:
77 Bio - Labtrade do Brasil
Subtotal: R$2.270,00
Biologia Molecular, NovaBio, Purificação
ExoSAP-IT PCR
Availability:
Fora de estoque
ExoSAP-IT ™ – PCR Product Cleanup Reagent
Reagente de limpeza do produto de PCR
R$400,00 – R$35.900,00
Conserva amostras de PCR – recuperação de 100% de produtos de PCR curtos e longos
Limpeza por PCR de um tubo / uma etapa – Adicione o reagente ExoSAP-IT® diretamente ao produto de PCR
Elimina colunas de rotação – diminui o tempo e as despesas enquanto aumenta o rendimento
Remove primers contaminantes e dNTPs – Nenhuma interferência em aplicações a jusante
Escalável – Econômico para purificação de alto rendimento
Limpa, elimina, remove e é escalável com processo simples e compatível com robôs,substitui grânulos, filtrações e placas
Processamento simples – compatível com robôs; Substitui grânulos, filtrações e placas Gera menos desperdício do que colunas – o reagente ExoSAP-IT foi projetado para limpeza simples e rápida de PCR para aplicações a jusante, como sequenciamento de DNA ou análise SNP (Single Nucleotide Polymorphism). Quando a amplificação por PCR estiver concluída, quaisquer dNTPs e iniciadores não consumidos restantes na mistura de produtos de PCR interferirão com esses métodos. O ExoSAP-IT remove esses contaminantes.
Conserves PCR samples — 100% recovery of both short and long PCR products
One tube/one step PCR cleanup — Add ExoSAP-IT® reagent directly to PCR product
Eliminates spin columns — Decreases time and expense while increasing yield
Removes contaminating primers and dNTPs — No interference in downstream applications
Scalable — Economical for high-throughput purification
Simple processing — Robotic-friendly; Replaces beads, filtrations, and plates
Generates less waste than columns – ExoSAP-IT reagent is designed for simple, quick PCR cleanup for downstream applications, such as DNA sequencing or Single Nucleotide Polymorphism (SNP) analysis. When PCR amplification is complete, any unconsumed dNTPs and primers remaining in the PCR product mixture will interfere with these methods. ExoSAP-IT removes these contaminants.Aditional InformationExoSAP-IT is added directly to the PCR product and incubated at 37°C for 15 minutes (Fig. 1). After PCR treatment, ExoSAP-IT is inactivated simply by heating to 80°C for 15 minutes.Fig. 1. Summary of ExoSAP-IT PCR product treatment.
ExoSAP-IT single-step PCR cleanup utilizes two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase (SAP), together in a specially formulated buffer, to remove unwanted dNTPs and primers from PCR products. Exonuclease I removes residual single-stranded primers and any extraneous single-stranded DNA produced in the PCR. SAP removes the remaining dNTPs from the PCR mixture.
Rapid PCR Product Cleanup Protocol
ExoSAP-IT requires only one pipetting step and two incubations. Just add ExoSAP-IT to the PCR product and within 30 minutes sequencing or SNP analysis can be performed.Simple: Single-Step
The method is designed to require a minimum of ‘hands-on’ time. Enzymatic removal of excess primers and unincorporated nucleotides occurs in one easy step by using ExoSAP-IT reagent in a single tube or microtiter well. Only simple pipette transfers are required, therefore, many samples can be processed at once, either manually or with robotics.No Sample Loss
Use of ExoSAP-IT reagent eliminates all gel or column purifications, sedimentations, filtrations, beads, and/or magnetic separations(1). There is 100% recovery of both short and long PCR products with ExoSAP-IT (Fig. 2).View Hi-Res PDF Version
Fig. 2. ExoSAP-IT treatment of PCR products with no sample loss. Single-copy targets were amplified from human genomic DNA. HES-1 (125 bp), numb (455 bp), NRAGE (1.55 kb), and numb (4.6 kb) were loaded on a 1.5% agarose gel before (pre) and after (post) ExoSAP-IT treatment. M is the DNA marker lane. Note that a variety of PCR product sizes can be treated with ExoSAP-IT, even a 125 bp fragment, with no sample loss.
Achieve High Data Quality from PCR Products
ExoSAP-IT reagent may be used as an effective cleanup method prior to fluorescent or radioactive DNA sequencing, SNP analysis, or any other application requiring a PCR product free of excess nucleotides and primers.
One tube/one step PCR cleanup — Add ExoSAP-IT® reagent directly to PCR product
Eliminates spin columns — Decreases time and expense while increasing yield
Removes contaminating primers and dNTPs — No interference in downstream applications
Scalable — Economical for high-throughput purification
Simple processing — Robotic-friendly; Replaces beads, filtrations, and plates
Generates less waste than columns – ExoSAP-IT reagent is designed for simple, quick PCR cleanup for downstream applications, such as DNA sequencing or Single Nucleotide Polymorphism (SNP) analysis. When PCR amplification is complete, any unconsumed dNTPs and primers remaining in the PCR product mixture will interfere with these methods. ExoSAP-IT removes these contaminants.Aditional InformationExoSAP-IT is added directly to the PCR product and incubated at 37°C for 15 minutes (Fig. 1). After PCR treatment, ExoSAP-IT is inactivated simply by heating to 80°C for 15 minutes.Fig. 1. Summary of ExoSAP-IT PCR product treatment.
ExoSAP-IT single-step PCR cleanup utilizes two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase (SAP), together in a specially formulated buffer, to remove unwanted dNTPs and primers from PCR products. Exonuclease I removes residual single-stranded primers and any extraneous single-stranded DNA produced in the PCR. SAP removes the remaining dNTPs from the PCR mixture.
Rapid PCR Product Cleanup Protocol
ExoSAP-IT requires only one pipetting step and two incubations. Just add ExoSAP-IT to the PCR product and within 30 minutes sequencing or SNP analysis can be performed.Simple: Single-Step
The method is designed to require a minimum of ‘hands-on’ time. Enzymatic removal of excess primers and unincorporated nucleotides occurs in one easy step by using ExoSAP-IT reagent in a single tube or microtiter well. Only simple pipette transfers are required, therefore, many samples can be processed at once, either manually or with robotics.No Sample Loss
Use of ExoSAP-IT reagent eliminates all gel or column purifications, sedimentations, filtrations, beads, and/or magnetic separations(1). There is 100% recovery of both short and long PCR products with ExoSAP-IT (Fig. 2).View Hi-Res PDF Version
Fig. 2. ExoSAP-IT treatment of PCR products with no sample loss. Single-copy targets were amplified from human genomic DNA. HES-1 (125 bp), numb (455 bp), NRAGE (1.55 kb), and numb (4.6 kb) were loaded on a 1.5% agarose gel before (pre) and after (post) ExoSAP-IT treatment. M is the DNA marker lane. Note that a variety of PCR product sizes can be treated with ExoSAP-IT, even a 125 bp fragment, with no sample loss.
Achieve High Data Quality from PCR Products
ExoSAP-IT reagent may be used as an effective cleanup method prior to fluorescent or radioactive DNA sequencing, SNP analysis, or any other application requiring a PCR product free of excess nucleotides and primers.
References:
DUGAN, K. A., LAWRENCE, H. S., HARES, D. R., FISHER, C. L. AND BUDOWLE B. (2002) J. Forensic Sci 47, 811-818.
HANKE, M. AND WINK, M. (1994) BioTechniques 17, 858-860.
MU, J., DUAN, J., MAKOVA, K., JOY, D., HUYNH, C., BRANCH, O., LI, W. AND SU, X. (2002) Nature 418, 323-326.
SILVA, JR., W. A., COSTA, M. C. R., VALENTE, V., DE FREITAS SOUSA, J., PACÓ-LARSON, M. L., ESPREAFICO, E. M., CAMARGO, S. S., MONTEIRO, E., DE JESUS, A., HOLANDA, M. A., ZAGO, M. A., SIMPSON, A. J. G. AND NETO, E. D. (2001) BioTechniques 30, 537-542.
WERLE, E., SCNEIDER C., RENNER, M., VÖLKER, M. AND FIEHN, W. (1994) Nucleic Acids Res. 22, 4354-4355.
Storage:
Shipped on dry ice. Store at -20°C.
| Código # | Description | Quantidade | Preço (USD) |
Preços (R$) |
| 78250 40 UL | ExoSAP-IT PCR Product Cleanup | 20 reactions | $38.00 | R$ 400,00 |
| 78200 200 UL | ExoSAP-IT PCR Product Cleanup | 100 reactions | $106.00 | R$ 1.130,00 |
| 78201 1 ML | ExoSAP-IT PCR Product Cleanup | 500 reactions | $413.00 | R$ 4.400,00 |
| 78202 4X1 ML | ExoSAP-IT PCR Product Cleanup | 2000 reactions | $1,483.00 | R$ 15.800,00 |
| 78205 10 ML | ExoSAP-IT PCR Product Cleanup | 5000 reactions | $3,370.00 | R$ 35.900,00 |
• Disponibilidade: produto feito sob encomenda
Shipping Policy
Prazo estipulado pelos Correios do Brasil.
Entrega de produtos perecíveis (-20 graus) ou (2 a 8 graus) é através de transportadora especializada.
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Biologia Molecular, Descontaminante, Descontaminantes, NovaBio, Promoção, Saneantes
Cleaner – Solução Aquosa inibidora de ácidos nucléicos – 250mL 14-0001-02.
Biologia Molecular, Descontaminante, Descontaminantes, NovaBio, Promoção, SaneantesCleaner – Solução Aquosa inibidora de ácidos nucléicos – 250mL 14-0001-02.
O Cleaner é uma solução descontaminante altamente eficaz na remoção de moléculas residuais de RNA/DNA/amplicons/resíduos de proteínas em superfícies laboratoriais, preservando a qualidade das amostras manipuladas na pesquisa.
SKU: 14-0001-02
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